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  • 您现在的位置:首页> 外文会议>Conference on Advanced Environmental, Chemical and Biological Sensing Technologies; 20070910-11; Boston,MA(US) >亿博帐号注册详情

    【24h】 Detection of Nitrite by flow injection analysis using a novel Paired Emitter-Detector Diode (PEDD) as a photometric Detector

    机译使用新型成对的发射极-检测器二极管(PEDD)作为光度检测器,通过流动注射分析法检测亚硝酸盐

    【摘要】An inexpensive flow injection analysis system for determining low concentration levels of nitrite employing the Griess reagent spectrophotometric method is reported. The novel photometric detector applied within this manifold is a highly sensitive, low cost, miniaturized light emitting diode (LED) based flow detector. This colorimetric detector employs two LEDs, operating one as a light source and the other as a light detector. The emitter LED is forward biased and the detector reverse biased. The emitter and detector LED had a λ_(max) of 530 nm and 623 nm respectively. The emission spectrum of the emitter LED efficiently overlapped with the absorbance spectrum of 9 μM NO_2 and Griess reagent complex. A simple timer circuit measures the time taken for the photocurrent generated by the emitter LED to discharge the detector LED from 5 V (logic 1) to 1.7 V (logic 0). The Griess reagent method employed for nitrite determination is based on the formation of an azo dye, the intensity of which is directly related to nitrite concentration. The linear range, reproducibility and limit of detection were investigated. Detection limits in the nanomolar range were achieved using the Paired Emitter-Detector Diode (PEDD) flow analysis device. For a comparative study the linear range and limit of detection were also investigated using a platewell reader. Higher sensitivity and improved precision were obtained from the PEDD compared to the commercially available plate well reader.

    【摘要机译】报道了使用格里斯试剂分光光度法测定低浓度亚硝酸盐的廉价流动注射分析系统。应用于该歧管的新型光度检测器是一种基于高灵敏度,低成本,小型化的发光二极管(LED)的流量检测器。该比色检测器采用两个LED,一个LED作为光源,另一个LED作为光探测器。发射器LED正向偏置,检测器反向偏置。发射器和检测器LED的λ_(max)分别为530 nm和623 nm。发射器LED的发射光谱与9μMNO_2和Griess试剂配合物的吸收光谱有效重叠。一个简单的计时器电路测量发射器LED产生的光电流使检测器LED从5 V(逻辑1)放电到1.7 V(逻辑0)所需的时间。用于亚硝酸盐测定的格里斯试剂法基于偶氮染料的形成,其强度与亚硝酸盐浓度直接相关。研究了线性范围,重现性和检测极限。使用配对的发射极-检测器二极管(PEDD)流量分析设备可达到纳摩尔级的检测限。为了进行比较研究,还使用板孔读数器研究了线性范围和检测限。与市售的酶标仪相比,PEDD具有更高的灵敏度和更高的精度。

    【原文格式】PDF

    机译 发光二极管;光学传感;亚硝酸盐检测;砂砾反应和流动分析;
  • 相关亿博帐号注册
  • 1. Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector [O ], O'Toole, Martina;  Shepherd, Roderick;  Lau, King-Tong;  Diamond, Dermot ...

    机译使用新型成对发射器 - 检测器二极管(pEDD)作为光度检测器,通过流动注射分析检测亚硝酸盐

  • 2. Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector [O ], O'Toole, Martina;  Shepherd, Roderick;  Lau, King-Tong;  Diamond, Dermot ...

    机译使用新型成对发射器 - 检测器二极管(pEDD)作为光度检测器,通过流动注射分析检测亚硝酸盐

  • 3. Determination of phosphate using a highly sensitive paired emitter-detector diode photometric flow detector [O ], O'Toole, Martina;  Lau, King-Tong;  Shepherd, Roderick;  Slater, Conor;  Diamond, Dermot ...

    机译使用高灵敏度配对的发射器 - 检测器二极管光度流量检测器测定磷酸盐

  • 4. Determination of Phosphate using a Highly Sensitive Paired Emitter-Detector Diode Photometric Flow Detector [O ], Martina O Toole;  King Tong Lau;  Roderick Shepherd;  Conor Slater ...

    机译用高灵敏度配对发射极 - 探测器二极管光度流动探测器测定磷酸盐

  • 5. Determination of phosphate using a highly sensitive paired emitter-detector diode photometric flow detector [O ], O'Toole, Martina;  Lau, King-Tong;  Shepherd, Roderick;  Slater, Conor;  Diamond, Dermot ...

    机译使用高灵敏度配对的发射器 - 检测器二极管光度流量检测器测定磷酸盐

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