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    【6h】Insights in two plasmid families from the SIO pier metagenome.

    机译来自SIO码头元基因组的两个质粒家族的见解。

    【摘要】From coastal California seawater, a complex and diverse environment, the marine cyanobacteria of the genus Synechococcus were enriched by flow cytometry-based sorting and the population metagenome was analyzed with 454 sequencing technology. Interestingly, at least three distinct mobile DNA elements not found in model Synechococcus strain genomes were detected in the assembled contigs. These contigs were confirmed to be plasmids. p27638e, p31635e, and p31454e, were sequenced and described. p27638e, p31635e, and p314543e are 1425, 1558, and 1479 kb in length, respectively. Further analysis of natural samples has shed light on their diversity over time. A recombinant plasmid, pTOPO27638 was constructed for sequencing and development of a shuttle vector capable of transforming both Escherichia coli and Synechococcus CC9311. Sixteen presumptive ORFs were identified which may code small peptides. The deduced amino acid sequence of the largest ORFs were significantly similar to that of a putative plasmid replication, repA. Comparison of the replication proteins from p27638e, p31635e and p31454e to other plasmid replication proteins show that it may replicate via rolling circle replication. p27638e and p31635e were found to be positively correlated with marine Synechococcus over time in the environment.

    【摘要机译】来自加利福尼亚沿海的海水,一个复杂多样的环境,通过流式细胞仪进行分选,富集了Synechococcus属的海洋蓝细菌,并使用454测序技术对种群的基因组进行了分析。有趣的是,在组装的重叠群中检测到了模型Synechococcus菌株基因组中未发现的至少三个不同的可移动DNA元件。这些重叠群被证实是质粒。对p27638e,p31635e和p31454e进行了测序和描述。 p27638e,p31635e和p314543e的长度分别为1425、1558和1479 kb。对天然样品的进一步分析揭示了其随时间的多样性。构建重组质粒pTOPO27638,用于测序和开发能够同时转化大肠杆菌和Syechechococcus CC9311的穿梭载体。鉴定出十六种可能编码小肽的ORF。推导的最大ORF的氨基酸序列与推定的质粒复制repA的氨基酸序列显着相似。从p27638e,p31635e和p31454e复制的蛋白质与其他质粒复制蛋白质的比较表明,它可以通过滚环复制进行复制。在环境中,p27638e和p31635e与海洋Synechococcus呈正相关。

    【作者】Latham, Elizabeth Anne.;

    【作者单位】University of California, San Diego.;

    【年(卷),期】2010(),

    【年度】2010

    【页码】67 p.

    【总页数】67

    【原文格式】PDF

    【正文语种】eng

    【中图分类】;

    【关键词】

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